Home
overview

With the ever-expanding therapeutic market, research and development is fundamental for improving current treatments and the design of protein based drugs of the future. With the variety of protein expression systems rapidly evolving, there is more pressure to harvest larger, purer quantities of product. Furthermore, a vast number of biomarker discovery studies have recently been embarked upon, requiring new, more efficient methods to identify and analyse proteins involved in disease populations with the aim to lead to novel diagnostic tools and treatments.

SMi’s Global Protein Summit, 2006 will be an excellent opportunity to hear about the latest developments from world leaders, bringing together expertise in effective protein expression, purification, identification and functional analysis and how to overcome the common and not so common pitfalls. Discussing research and manufacturing activities in both academia and industry, this unique opportunity will provide genuine opportunities of networking. A must attend event for all those involved in protein research and production.

Hear contributions from leading industry experts, including:

  • Dr Scott Patterson, Senior Director, Medical Sciences, Amgen Inc
  • Dr Zhijian Lu, Assistant Director, Biotherapeutics Expression & Purification, Wyeth
  • Dr Stephen Irving, Senior Principal Scientist, Pfizer
  • Dr Joey M. Studts, Principal Scientist & Head, Protein Resources, Boehringer-Ingelheim
  • Dr Thierry Battle, Biotechnology Services Manager, Serono Pharmaceutical Research Institute
  • Dr Tauseef Butt, President, Life Sensors
  • Dr Robert Wynands, Director, Structural Biology, Takeda San Diego
  • Dr Alex Breeze, Head, NMR Structural Biology, AstraZeneca
  • Dr Michael Dyson, Project Leader, Protein Expression, The Wellcome Trust Sanger Institute

Conference programme

8:30 Registration & Coffee

9:00 Chairman's Opening Remarks

Tauseef Butt

Tauseef Butt, President, Life Sensors

9:10 MOVING PROTEIN DRUG CANDIDATES FROM DISCOVERY TO DEVELOPMENT

Zhijian Lu

Zhijian Lu, Assistant Director, Biotherapeutics Expression & Purification, Wyeth Research

  • The biotech heritage of Wyeth
  • Different forms of recombinant protein drugs
  • Protein expression in E. coli, thioredoxin fusion system
  • CHO cell expression system
  • Process improvement
  • 9:50 SUMO TAGS AND DIFFICULT TO EXPRESS PROTEINS

    Tauseef Butt

    Tauseef Butt, President, Life Sensors

  • SUMOylation system, nature’s design for tagging and chaperoning proteins in cells
  • SUMO family of tags remarkably enhance protein expression and facilitate purification
  • No parallel system for producing proteins with desired N-termini
  • Chaperone properties of SUMO system to produce native structures
  • Designer tags for solubilisation of challenging proteins
  • 10:30 Morning Coffee

    11:30 INSECT CELL EXPRESSION SYSTEMS

    Joey Studts

    Joey Studts, Principal Scientist & Head, Protein Resources, Boehringer Ingelheim

  • Baculovirus instead of E.coli expression as first line trials for structural research support
  • Elimination of cloning bottleneck using gene synthesis collaboration
  • Multi-paralleled expression screening of constructs on an E.coli timescale
  • 4 days form DNA to expression results
  • 21 days from DNA to Biomass, including plaque purification
  • Reproducible scale-up without the use of viral titres
  • 12:10 A SIMPLE TECHNOLOGY FOR THE SIMULTANEOUS EXPRESSION OF 4 TANDEM GENES

    Jon Chestnut

    Jon Chestnut, Research & Development, Invitrogen Ltd

  • Expression of multiple proteins from one promoter or multiple proteins with different promoters
  • Protein expressed at desired levels in the same cell
  • Eliminating the need for co-transfection of multiple plasmids
  • Expression of 1-4 different inserts from one vector
  • Ultimate flexibility: removal and replacement of single or multiple inserts from a construct
  • 12:50 Networking Lunch

    14:20 HIGH-THROUGHPUT PROTEIN PRODUCTION SYSTEM AT SMALL / INTERMEDIATE SCALE

    Thierry Battle

    Thierry Battle, Biotechnology Services Manager, Serono Pharmaceutical Research Institute

  • Summary of general pipeline system and mechanistics
  • Lessons learned
  • A discussion of issues regarding tech-transfer
  • 15:00 BACULOVIRUS PRODUCTION AND PROTEIN EXPRESSION

    Kevin Richards

    Kevin Richards, Senior scientist, NextGen Sciences Ltd

  • An overview of traditional methods
  • New technologies to overcome the labour intensive and technically demanding nature of baculovirus production and protein expression
  • Development of the baculoworkstation
  • Manual vs automated procedures
  • The advantages of the baculoworkstation
  • 15:40 Afternoon Tea

    16:00 PRODUCTION OF SOLUBLE MAMMALIAN PROTEINS IN ESCHERICHIA COLI

    Michael Dyson

    Michael Dyson, Project Leader, Protein Expression, Wellcome Trust Sanger Institute

  • Cloning strategy
  • Comparison of tags and host strains for E. coli expression
  • Production pipeline to supply antigens for antibody production
  • A case study: the immunoglobulin superfamily (IgSF)
  • 16:40 HIGH THROUGHPUT VALUATION OF PROTEIN EXPRESSION CLONES

    Konrad Bussow

    Konrad Bussow, Team Leader, Protein Structure Factory, Max Planck Institute for Molecular Genetics

  • E. coli protein expression and protein purification in microtitre plates
  • Comparison of micro-scale and large-scale protein production for crystallisation and structural analysis
  • Data management of large numbers of clones
  • 17:20 Chairman’s Closing Remarks and Close of Day One

    8:30 Registration & Coffee

    9:00 Chairman's Opening Remarks

    Scott Patterson

    Scott Patterson, Senior director, Medical Sciences, Amgen

    9:10 BIOMARKERS – THE NEW AGE OF DIAGNOSTICS AND DRUG DISCOVERY?

    Scott Patterson

    Scott Patterson, Senior director, Medical Sciences, Amgen

  • Identifying the unmet medical need
  • Techniques and tools
  • New developments
  • Potential pitfalls in biomarker analysis
  • 9:50 PROTEIN PROCESSING

    Paul Docherty

    Paul Docherty, Buiness Development, Novexin

  • Greater protein stability
  • Minimise losses during purification
  • Obtain full structural data without interference with the protein
  • Simpler protein refolding
  • Better protein assays and analysis
  • 10:30 Morning Coffee

    10:55 HIGH THROUGHPUT PROTEIN PURIFICATION

    David Bannister

    David Bannister, Senior Protein Purification Scientist, Cambridge Antibody Technology

  • Overview of high throughput protein purification methods and hardware
  • Purity vs yield
  • Time and cost implications
  • The future of protein purification
  • 11:30 PROTEIN PURIFICATION AND CRYSTALLISATION SUCCESS RATES

    Robert  Wynands

    Robert Wynands, Director, Structural Biology, Takeda San Diego, Inc

  • High throughput protein purification methods used to produce proteins for crystallisation trials
  • Analytical methods used in-process and for end products
  • Nanovolume Crystallisation®
  • The use of databases for tracking
  • Examples
  • Lessons learnt

  • 12:40 Networking Lunch

    13:40 STRATEGIES FOR SUPPLYING PROTEINS FOR STRUCTURAL STUDIES

    Stephen Irving

    Stephen Irving, Senior Principal Scientist, Pfizer

  • Protein production – overcoming challenges
  • Experiences with alternative expression systems
  • Protein purification and affinity tags
  • Mutagenisis strategies
  • Case studies
  • 14:20 PROTEIN REFOLDING

    Stefan Schmidt

    Stefan Schmidt, Team Leader, Protein Science, AstraZeneca

  • Current strategies
  • Optimising conditions
  • Automated and parallel approaches
  • Functional analysis
  • Challenges and future perspectives
  • 15:00 THE NEXT FRONTIER IN PROTEIN ANALYSIS

    Bahram Fathollahi

    Bahram Fathollahi, Research & Development Engineer, Microfluidics, Caliper Life Sciences

  • Time to result and quantitative data are vital in protein process decisions
  • Quality of decisions are based on quality of data
  • Microfluidic assays are the new SDS-PAGE
  • Automated analysis is key to data reproducibility and laboratory throughput
  • 15:40 Afternoon Tea

    16:40 FRAGMENT-BASED DRUG DISCOVERY

    Alex  Breeze

    Alex Breeze, Head, NMR Structural Biology, AstraZeneca

  • Why screen fragments?
  • NMR-based approaches for fragment library screening
  • Challenges for crystallography of fragment hits
  • Building leads from fragments
  • Embedding fragment-based approaches into the big pharma environment
  • Case studies from AstraZeneca
  • 17:20 Chairman’s Closing Remarks and Close of Day One

    +

    Workshops

    Protein Expression Technologies
    Workshop

    Protein Expression Technologies

    Holiday Inn Bloomsbury
    9th June 2006
    London, United Kingdom

    Domains: Drug Targets & Vaccines
    Workshop

    Domains: Drug Targets & Vaccines

    Holiday Inn Bloomsbury
    9th June 2006
    London, United Kingdom

    Holiday Inn Bloomsbury

    Coram Street
    London WC1N 1HT
    United Kingdom

    Holiday Inn Bloomsbury

    Holiday Inn London-Bloomsbury is just 2 minutes' walk from the Tube at Russell Square station, for speedy connections across the capital. We're 10 minutes by bus from Eurostar connections at St Pancras station, and a 45-minute taxi ride from London Heathrow Airport. Check into your stylish Guest room, air-conditioned and connected to wireless Internet.

    HOTEL BOOKING FORM

    Title

    SubTitle
    speaker image

    Content


    Title


    Description

    Download


    WHAT IS CPD?

    CPD stands for Continuing Professional Development’. It is essentially a philosophy, which maintains that in order to be effective, learning should be organised and structured. The most common definition is:

    ‘A commitment to structured skills and knowledge enhancement for Personal or Professional competence’

    CPD is a common requirement of individual membership with professional bodies and Institutes. Increasingly, employers also expect their staff to undertake regular CPD activities.

    Undertaken over a period of time, CPD ensures that educational qualifications do not become obsolete, and allows for best practice and professional standards to be upheld.

    CPD can be undertaken through a variety of learning activities including instructor led training courses, seminars and conferences, e:learning modules or structured reading.

    CPD AND PROFESSIONAL INSTITUTES

    There are approximately 470 institutes in the UK across all industry sectors, with a collective membership of circa 4 million professionals, and they all expect their members to undertake CPD.

    For some institutes undertaking CPD is mandatory e.g. accountancy and law, and linked to a licence to practice, for others it’s obligatory. By ensuring that their members undertake CPD, the professional bodies seek to ensure that professional standards, legislative awareness and ethical practices are maintained.

    CPD Schemes often run over the period of a year and the institutes generally provide online tools for their members to record and reflect on their CPD activities.

    TYPICAL CPD SCHEMES AND RECORDING OF CPD (CPD points and hours)

    Professional bodies and Institutes CPD schemes are either structured as ‘Input’ or ‘Output’ based.

    ‘Input’ based schemes list a precise number of CPD hours that individuals must achieve within a given time period. These schemes can also use different ‘currencies’ such as points, merits, units or credits, where an individual must accumulate the number required. These currencies are usually based on time i.e. 1 CPD point = 1 hour of learning.

    ‘Output’ based schemes are learner centred. They require individuals to set learning goals that align to professional competencies, or personal development objectives. These schemes also list different ways to achieve the learning goals e.g. training courses, seminars or e:learning, which enables an individual to complete their CPD through their preferred mode of learning.

    The majority of Input and Output based schemes actively encourage individuals to seek appropriate CPD activities independently.

    As a formal provider of CPD certified activities, SMI Group can provide an indication of the learning benefit gained and the typical completion. However, it is ultimately the responsibility of the delegate to evaluate their learning, and record it correctly in line with their professional body’s or employers requirements.

    GLOBAL CPD

    Increasingly, international and emerging markets are ‘professionalising’ their workforces and looking to the UK to benchmark educational standards. The undertaking of CPD is now increasingly expected of any individual employed within today’s global marketplace.

    CPD Certificates

    We can provide a certificate for all our accredited events. To request a CPD certificate for a conference , workshop, master classes you have attended please email events@smi-online.co.uk

    Event Title

    Headline

    Text
    Read More

    I would like to speak at an event

    I would like to attend an event

    I would like to sponsor/exhibit at an event

    SIGN UP OR LOGIN

    Sign up
    Forgotten Password?

    Contact SMi GROUP LTD

    UK Office
    Opening Hours: 9.00 - 17.30 (local time)
    SMi Group Ltd, 1 Westminster Bridge Road, London, SE1 7XW, United Kingdom
    Tel: +44 (0) 20 7827 6000 Fax: +44 (0) 20 7827 6001
    Website: http://www.smi-online.co.uk Email: events@smi-online.co.uk
    Registered in England No: 3779287 VAT No: GB 976 2951 71




    Forgotten Password

    Please enter the email address you registered with. We will email you a new password.