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3D YOU CAN SEE – PLANAR 3D SPHEROID ARRAYS FOR HISTOLOGY
February 19th, 2019

Certain standard procedures are indispensable for life sciences. Histology is one of them. Now, with 3D spheroids, a model to closely mimic human physiology for a vast array of applications, is finally broadly available. But for 3D spheroids, histological assessment so far has been done as irregular bulk without a clear optical plane.
Thanks to the totally regular array spheroid building in the Sphericalplate 5D, we will show how to catch spheroids with polymerizing gel and processing histology to a planar array of 750 spheroids. This perfectly fits a microscope slide and allows the user to do histological assessment of 3D spheroids like never before.

Workshop Leaders:
Patrick Kugelmeier, Founder, Director of Science, Kugelmeiers AG
Michael Raghunath
, Professor for Biochemistry, Protein Technology and Tissue Engineering, Zurich University of Applied Sciences

Patrick Kugelmeier studied medicine and did his medical thesis in islet transplantation for the treatment of Diabetes. The joy of research led to an MD-PhD program for the early differentiation of stem cells. Cell culture technologies at that time didn't allow for good data reproducibility, because stem cells were cultured in 2D. Fueled by the clinical need of small, size-controlled cell clusters to improve islet transplantation and the insights from the sensitivity of stem cells, he began to develop what was later to become the Sphericalplate 5D. After the MD-PhD program, he did his clinical training in visceral and transplant surgery followed by trauma surgery. Besides the clinical work, the Sphericalplate 5D was further developed. Finally, it became clear that this technology opens a new dimension in cell transplantation and regenerative medicine and Dr. Kugelmeier decided to quit surgery to fully live this vision within a startup-company, the Kugelmeiers AG.

Kugelmeiers Ltd. is a Spin-off company from the University of Zurich, Switzerland founded in 2015 with a team of medical, research and transplant specialists and serial entrepreneurs. It develops and markets 3D cell culture technologies and advances stem cell therapies for future-oriented medical applications. The first series product, the Sphericalplate 5D, allows the translational production of hundreds to millions of regular cell spheroids in clinical quality. In 2019, the clinical study for the approval of the Sphericalplate 5D as a medical device for cell transplantation begins.

Michael Raghunath is a clinical scientist and internationally renowned for work in matrix biology, wound healing, and implementing the biophysical principle of macromolecular crowding in tissue engineering. After completing medical studies in Mainz, Germany, he received training in immunology at the University of Heidelberg, Germany, and as a postdoc in matrix biology and wound healing at the University of Zurich, Switzerland. In 1997, he received his habilitation in Physiological Chemistry and Pathobiochemistry at the University of Münster, Germany, and did a clinical residency in dermatology there. After a stint in industry as R&D director, he became an Associate Professor of Biomedical Engineering and Biochemistry in 2003 at the National University of Singapore. In 2016, he joined ZHAW as Professor and Head, Center of Cell Biology & Tissue Engineering. He currently holds 123 research publications (h-index: 42; 4813 cites) and several review articles and book chapters. He is member of the EU Council for TERMIS and in the editorial board of Current Opinion in Biomedical Engineering, Journal of Tissue Engineering and Regenerative Medicine, and International Journal of Bioprinting. As Director of the Competence Center for Tissue Engineering (TEDD) he has a strong interest in 3D tissue models to replace animal experimentation.

The ZHAW is one of the leading Swiss universities of applied sciences. Around 1,500 students are currently enrolled at the School of Life sciences and facility Management, which as more than 600 employees. We offer five Bachelor’s and three Master’s degree programmes, together with a wide range of continuing education courses.
Our expertise in life sciences and facility management in the areas of the environment, food and health enables us to make a vital contribution ot solving social challenges and improving quality of life. Our success is based on five dynamic institutes with extensive competence in research, development and services in the disciplines of chemistry and biotechnology, food and beverage innovation, natural resource sciences, applied simulation and facility management.
 

  • If you do histology on 3D spheroids, this workshop is for you. It offers you a new dimension of scalability and analysis flow.
  • Various research areas like cancer screening and stem cell research need an individual understanding of spheroid biology – cut in one plane x 750 samples gives you unparalleled data accuracy.
  • Statistical power will be much higher, as you can rely on more, regular data points.
  • Various showcases like hypoxia effects on spheroid biology or cell population heterogeneity can be studied more accurately when spheroids are organised in one plane.

Workshop programme

8:30 Registration & Coffee

9:00 Chairman's Opening Remarks

9:10 The spheroidal world of cell culture

Patrick Kugelmeier

Patrick Kugelmeier, Director of Science, Kugelmeiers
View Bio

  • Why 3D, why spheroids
  • Current methods to make spheroids, pro’s and cons
  • 9:50 Applications and impact

    Patrick Kugelmeier

    Patrick Kugelmeier, Director of Science, Kugelmeiers
    View Bio

  • Spheroid Transplantation – the workhorse of regenerative medicine
  • Applied: modified islet transplantation analysis
  • Stem cell spheroid research
  • Cancer research
  • 10:30 Morning Coffee

    11:00 Planar spheroid arrays

    Michael  Raghunath

    Michael Raghunath, Head Center for Cell Biology & Tissue Engineering Director Competence Center TEDD, Zurich University of Applied Sciences
    View Bio

  • The principle of planar spheroid arrays and the basic idea
  • Generating planar spheroid array from multiplates
  • Trouble-shooting and challenges
  • 11:40 Hands-on: trials: make your own array, look at sections

    Michael  Raghunath

    Michael Raghunath, Head Center for Cell Biology & Tissue Engineering Director Competence Center TEDD, Zurich University of Applied Sciences
    View Bio

  • Agarose embedding, array retrieval, processing
  • Discussion round
  • 12:20 Closing Remarks

    12:30 End of Workshop

    +

    FEATURED SPEAKERS

    Michael  Raghunath

    Michael Raghunath

    Head Center for Cell Biology & Tissue Engineering Director Competence Center TEDD, Zurich University of Applied Sciences
    Patrick Kugelmeier

    Patrick Kugelmeier

    Director of Science, Kugelmeiers

    Michael Raghunath

    Head Center for Cell Biology & Tissue Engineering Director Competence Center TEDD, Zurich University of Applied Sciences
    Michael  Raghunath

    Michael Raghunath is a clinical scientist and internationally renowned for work in matrix biology, wound healing, and implementing the biophysical principle of macromolecular crowding in tissue engineering. After completing medical studies in Mainz, Germany, he received training in immunology at the University of Heidelberg, Germany, and as a postdoc in matrix biology and wound healing at the University of Zurich, Switzerland. In 1997, he received his habilitation in Physiological Chemistry and Pathobiochemistry at the University of Münster, Germany, and did a clinical residency in dermatology there. After a stint in industry as R&D director, he became an Associate Professor of Biomedical Engineering and Biochemistry in 2003 at the National University of Singapore. In 2016, he joined ZHAW as Professor and Head, Center of Cell Biology & Tissue Engineering. He currently holds 123 research publications (h-index: 42; 4813 cites) and several review articles and book chapters. https://scholar.google.com.sg/citations?user=siN1tiUAAAAJ&hl=en
    He is member of the EU Council for TERMIS and in the editorial board of Current Opinion in Biomedical Engineering, Journal of Tissue Engineering and Regenerative Medicine, and International Journal of Bioprinting. As Director of the Competence Center for Tissue Engineering (TEDD) he has a strong interest in 3D tissue models to replace animal experimentation.
     

    Patrick Kugelmeier

    Director of Science, Kugelmeiers
    Patrick Kugelmeier

    Patrick Kugelmeier studied medicine and did his medical thesis in islet transplantation for the treatment of Diabetes. The joy of research led to an MD-PhD program for the early differentiation of stem cells. Cell culture technologies at that time didn't allow for good data reproducibility, because stem cells were cultured in 2D. Fueled by the clinical need of small, size-controlled cell clusters to improve islet transplantation and the insights from the sensitivity of stem cells, he began to develop what was later to become the Sphericalplate 5D.
    After the MD-PhD program, he did his clinical training in visceral and transplant surgery followed by trauma surgery. Besides the clinical work, the Sphericalplate 5D was further developed. Finally, it became clear that this technology opens a new dimension in cell transplantation and regenerative medicine and Dr. Kugelmeier decided to quit surgery to fully live this vision within a startup-company, the Kugelmeiers AG.
     

    VENUE

    Copthorne Tara Hotel

    Scarsdale Place, Kensington, London, United Kingdom

    The Copthorne Tara Hotel London Kensington is an elegant contemporary four-star hotel in prestigious Kensington, located just a two minutes walk from High Street Kensington underground station, making exploring easy. The hotel offers well-appointed and comfortable guest rooms combining Standard, Superior and Club accommodation. Club rooms offer iconic views over the city and include Club Lounge access for complimentary breakfast and refreshments. Guests can sample the authentic Singaporean, Malaysian and Chinese cuisine at Bugis Street, traditional pub fare at the Brasserie Restaurant & Bar or relax with a delicious drink at West8 Cocktail Lounge & Bar.

    The Copthorne Tara Hotel boasts 745 square meters of flexible meeting space, consisting of the Shannon Suite and the Liffey Suite, ideal for hosting conferences, weddings and social events. Facilities include access to the business centre 24 hours a day, fully equipped fitness room, gift shop, theatre desk and Bureau de Change. With ample onsite parking outside the London congestion charge zone and excellent transport links via Heathrow Airport, the hotel is the perfect location for business or leisure stays. The hotel is within close proximity to the shops of High Street Kensington, Knightsbridge and Westfield London, Olympia Conference Centre, Royal Albert Hall, Kensington Palace and Hyde Park.

     

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    Copthorne Tara Hotel

    Scarsdale Place
    Kensington
    London W8 5SR
    United Kingdom

    Copthorne Tara Hotel

    The Copthorne Tara Hotel London Kensington is an elegant contemporary four-star hotel in prestigious Kensington, located just a two minutes walk from High Street Kensington underground station, making exploring easy. The hotel offers well-appointed and comfortable guest rooms combining Standard, Superior and Club accommodation. Club rooms offer iconic views over the city and include Club Lounge access for complimentary breakfast and refreshments. Guests can sample the authentic Singaporean, Malaysian and Chinese cuisine at Bugis Street, traditional pub fare at the Brasserie Restaurant & Bar or relax with a delicious drink at West8 Cocktail Lounge & Bar.

    The Copthorne Tara Hotel boasts 745 square meters of flexible meeting space, consisting of the Shannon Suite and the Liffey Suite, ideal for hosting conferences, weddings and social events. Facilities include access to the business centre 24 hours a day, fully equipped fitness room, gift shop, theatre desk and Bureau de Change. With ample onsite parking outside the London congestion charge zone and excellent transport links via Heathrow Airport, the hotel is the perfect location for business or leisure stays. The hotel is within close proximity to the shops of High Street Kensington, Knightsbridge and Westfield London, Olympia Conference Centre, Royal Albert Hall, Kensington Palace and Hyde Park.

     

    HOTEL BOOKING FORM

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    WHAT IS CPD?

    CPD stands for Continuing Professional Development’. It is essentially a philosophy, which maintains that in order to be effective, learning should be organised and structured. The most common definition is:

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